| Character | Principal | Ensemble |
| Alice | Lottie Heels | Nel Alldridge |
| Queen of Hearts | Joanna Hughes | Sharon Beal |
| White Rabbit | Pete Coxhead | Karen Davies |
| Duchess | Rob Brown | Sue Evans |
| Mad Hatter | Maria Stafford | Danielle Foster |
| March Hare | Cath Skyrme | Zoe Hickman |
| Dormouse | Laura Fullelove | Lotte Hunting |
| Cheshire Cat | Matthew Heels | Debbie Lane |
| Caterpillar | Ellie Johnson | NIcki Martin |
| Reggie | Charlie Green | Simone Pascolutti – Werrett |
| Ivy | Sarah Gilhooly | Rachael Satchwell |
| Tiger Lil | Claire Hemingway | Emma Smith |
| Daff | Jennifer Brinton | Sally Smith |
| Posey | Ella Holland | |
| Nanny | Helen Lowe | |
| King | Jason Beard | |
| Veronica – Sarah | ||
| Virginia – Claire | ||
| Victoria – Jennifer |
